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We have a SharePoint 2010 server on a domain machine. Now we try to login to that server with domain users using web service calls , However we are getting 401 error while logging in .We have checked that the windows authentication for the particular web application (IIS site ) is disabled , so we enabled it .. Are there any other reasons for which login fails ?

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Can the Domain User actually login to sharepoint, eg do they have access to Root site on the site collection?

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