If you include a file that does not exist with include_once, the return result will be false.
If you try to include that same file again with include_once the return value will be true.
Example:
<?php
var_dump(include_once 'fakefile.ext'); // bool(false)
var_dump(include_once 'fakefile.ext'); // bool(true)
?>
This is because according to php the file was already included once (even though it does not exist).